DNA Slot Blot Repair Assay - Bio-protocol

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dna slot Usually, 10–100 ng of DNA in the gel- loading buffer of choice is applied to a slot The gel is then run for 30–60 min at high voltage (5–20 V  Slot blotting DNA Danna Eickbush 1 Solutions Buffers needed: a M Tris, pH b 20X SSC c 2X Denaturing  A slot blot method, involving the use of T4 DNA polymerase-associated exonuclease digestion of uv-irradiated DNA, was used to detect

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